Serial Dilution Step Planner
Break down high-order serial dilutions into manageable steps by specifying the stock concentration, desired target, and per-step ratio.
Examples
- 1,000,000× stock to 100× with 1:10 steps, 1 mL aliquot ⇒ Result: 5 steps • each: 1.00 mL sample + 9.00 mL diluent
- 500× stock to 0.5× with 1:5 steps, 0.5 mL aliquot ⇒ Result: 4 steps • each: 0.50 mL sample + 2.00 mL diluent
FAQ
How do I handle volumes that exceed my tube size?
Reduce the aliquot volume and proportionally scale the diluent so the ratio remains constant.
Can I plan different final volumes?
Yes. Multiply the per-step volumes by the number of replicates needed or adjust the aliquot to deliver the desired final volume per tube.
What if I need an exact concentration between steps?
Perform the serial dilution up to the nearest higher concentration, then make a final partial dilution to fine tune the exact target.
Additional Information
- Outputs the number of dilution steps plus the sample and diluent volumes per step.
- The calculation assumes constant dilution factor at each stage.
- Adjust aliquot volume to match the pipettes or tubes available in your workflow.
- For uneven steps, round down the final aliquot and prepare an additional fine-tune dilution if required.