mRNA Vaccine Batch Yield Calculator

Translate plasmid loading, IVT efficiency, and downstream recovery into the purified mRNA mass available for filling or lipid nanoparticle encapsulation.

Mass of linearised plasmid template loaded into in vitro transcription.
Fraction of template converted into full-length mRNA during IVT.
Overall mass recovery through capture, polishing, and concentration steps.
Fraction lost to holds, filtration, or vial residuals. Defaults to 2% when blank.

Process development aid. Confirm yields with in-process analytics, validated assays, and GMP documentation before committing to supply agreements.

Examples

  • 150 mg template, 65% transcription, 80% purification, 2% fill loss ⇒ Bulk mRNA yield 0.08 g (76.44 mg) and 1,528 doses at 0.05 mg each.
  • 220 mg template, 72% transcription, 85% purification, fill loss left blank (defaults 2%) ⇒ Bulk mRNA yield 0.13 g (133.66 mg) and 2,673 doses.

FAQ

Why does the calculator request purification recovery separately?

Capture, enzymatic cleanup, and ultrafiltration each introduce stepwise losses. Tracking aggregate recovery ensures the yield projection reflects real-world downstream performance rather than theoretical IVT output.

How should I measure transcription efficiency?

Quantify full-length mRNA via HPLC, CE, or RT-qPCR against a calibrated standard curve immediately after IVT, and divide by the theoretical maximum determined from template mass and stoichiometry.

Can I change the dose size?

Yes. Multiply the reported milligram total by 1,000 to obtain micrograms, then divide by your dose requirement to derive batch-equivalent doses for alternate formulations.

Additional Information

  • Result unit: grams of purified mRNA plus equivalent milligrams and dose count.
  • Fill-finish loss defaults to 2% to reflect hold-up in filters and vials when left empty.
  • Dose equivalence assumes 0.05 mg per dose; adjust downstream documents if your formulation differs.

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